Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.

Purpose. To define spring barley breeding lines with an optimal combination of yielding capacity and stability under different weather conditions in the Central Forest-Steppe zone of Ukraine. Methods. Field studies, ANOVA, AMMI, GGE biplot analysis. Results. Hydrothermal regime during interphase periods of spring barley vegetation under conditions of The V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2012–2014 was characterized by significant variability, that facilitated detailed evaluation of the lines for productivity, stability, and resistance to abiotic and biotic factors. In the performance of an experiment, the highest average yielding capacity (5.87 t/ha) was noted in 2012, the lowest one (3.50 t/ha) was in 2013. As for these years, average yield of 4.63 t/ha was obtained in 2014. By applying AMMI and GGE biplot analysis, significant differences in response of the studied lines to variability of weather conditions was revealed. Using AMMI model, additive components of the main effects of the breeding lines and years of testing as well as multiplicative components of their interaction were characterized. GGE biplot genotypes ranking in relation to a hypothetical “ideal” genotype showed an absolute advantage of breeding line ‘Nutans 4540’ for yielding capacity and stability. In addition, breeding lines ‘Nutans 4241’ and ‘Nutans 4120’ were close to ideatype. Selected breeding lines were characterized by resistance and moderate resistance to leaf diseases and lodging. Conclusions. Use of AMMI and GGE biplot analysis to evaluate breeding lines at the final stages of breeding process allows to describe them thoroughly and graphically as well as differentiate them not only for average yielding capacity but also for their interaction with changing conditions during the years of testing. Breeding lines ‘Nutans 4540’, ‘Nutans 4241’, and ‘Nutans 4120’ to be identified for the stability of yielding capacity display in combination with other agronomic characters were transferred to the State variety testing as new spring barley varieties ‘MIP Myrnyi’, ‘MIP Saliut’, and ‘MIP Sotnyk’ respectively.

Purpose. To develop a tobacco variety model with optimal inflorescence traits such as size and shape that will allow to increase seed productivity of the crop. Methods. Statistical and mathematical (correlative, regressive) ones. Results. Basic collection consisting of 282 variety samples registered in the National Genetics Center was evaluated, optimal parameters of inflorescence were defined that can provide a high seed yield. During statistical analysis, correlation matrix was developed with the purpose to highlight traits that correlate with inflorescence productivity. According to the results of correlation analysis, a strong relationship between the width and height of inflorescence (r = 0,773±0,038) was established. Somewhat weaker correlation was observed when modeling regressive relation between inflorescence height and width, where regression showed the medium relationship. Regression equation of these traits is as follows: y = 0,5585x + 8,4649. Inflorescence density (r = 0,646), height (r = 0,556) and width (r = 0,527) also had quite a high positive effect on seed productivity. The results of regression analysis pointed to the fact that there were a linear relationship between inflorescence size and seed productivity. Conclusions. Among 282 samples of basic tobacco collection, 29 varieties with high seed productivity was defined which can be used in the breeding process, and ‘Sobolchskyi 15/21’, ‘Ergo 23’, ‘C-11’, ‘Sygarnyi 99’ varieties were selected for large-scale implementation into the production of raw material of cigar type.

Purpose. Analysis of the process of hexaploid triticale breeding for winter hardiness by intraspecific hybridization method using systemic ecological tests under contrasting conditions. Methods. Dialectical investigation, field experiments, laboratory testing and statistical evaluation. Results. The results of breeding winter and alternate triticale varieties possessing a complex of valuable traits by the method of intraspecific hybridization of forms of different types of development using systemic ecological tests under contrasting conditions (Forest-Steppe – extremely arid Steppe) and at low temperatures were presented. During the years of research (1980–2005), 18 varieties were developed and transferred to the state testing, 17 of them were registered. Conclusions. Thus, the effective selection of highly productive genotypes with increased and high winter hardiness is possible from populations obtained by crossing hexaploid triticale of different types of development (winter triticale with spring and alternate ones) and contrasting level of frost and winter hardiness. During the period of 1980–2015, medium-tall and dwarf varieties of winter (‘Amfidyploid 256’, ‘Garne’, ‘Kharroza’, ‘Rarytet’, ‘Timofey’, etc.) and alternate (‘Nikanor’, ‘Yaroslava’, ‘Plastun Volynskyi’) triticales were developed. They are superior to the standard varieties of soft winter wheat for the critical temperature of freezing by -0.5...- 2.0 °C, characterized by increased (up to 9–12 t/ha) grain yield of various quality depending on the purpose of use.

Purpose. To determine morphological and morphometric features of vegetative and generative organs of Persica species and varieties under the conditions of the Forest-Steppe zone of Ukraine for further use in breeding. Methods. Biological (morphological analysis) and statistical (processing of morphometric parameters) ones. Results. It was found that except ternate multiple buds, in peach hybrid ‘Suputnyk’collateral buds were prevalent which was not common to other tested varieties and species. Buds of each species and variety have a certain number of scales. The most scales were observed in ‘Suputnyk’. Pubescence of abaxial side of the leaf in P. dav?diana and ‘Suputnyk’ plants was not revealed. Species of P. vulgaris (‘Pecherskyi’, ‘Antotsianovyi’, ‘Poleskyi’) was characterized by increase in size of leaf blades, flowers and fruits. The distinctive features of P. davidiana and ‘Suputnyk’ are that stamens were accumulated in their flowers while in P. vulgaris they are branched. Raphe of a P. vulgaris stone protrudes above the edges of the bordering creases and consists of a number of narrow plates. Conclusions. For the breeding purposes, plants have an advantage for some basic criteria: the most flower buds that is common to interspecies hybrid ‘Suputnyk’; increase in photosynthetic productivity of the leaf apparatus to be typical for the variety ‘Pecherskyi’; large fruits that ‘Pecherskyi’ variety is noted for; intense red color of fruits that is inherent feature of ‘Antocianovyi’ variety.

Purpose. To establish the reasons of submitting not highly decorative lilac hybrids to state variety testing so that unpromising varieties and look-alikes can be sometimes included in the world collection, and focus on solving this situation. Methods. Analytical approach. Results. It was established why and how unoriginal hybrids were submitted to state variety testing that for several reasons can allow them to obtain the status of variety. Conclusions. In order to prevent obtaining the status of variety by some low decorative lilac hybrids, it is necessary that both a breeder and testing stations employer should use known presentable collections of identified varieties for evaluation of real value of a new hybrid. In Ukraine, there is such a collection at N. N. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine where more than 130 varieties and 21 species of the genus Syringa occupies the area of 2.35 ha. It is desirable to compare not only with the check variety that the breeder selected for his hybrid among such, but, if it is possible, with all varieties in the collection close to it by decorative effect. The problem is that the author of a hybrid, sometimes ignorantly or for some other reason, selects a check variety for his hybrid that is not the most decorative one among such, against which a candidate for variety status will be more attractive during its evaluation. In such a case, the breeder should change a check variety that permits to estimate really the decorative effect of a submitted hybrid.

Purpose. To analyze the Ukrainian names of the fruit crops which varieties are undergoing scientific and technical examination. Results. Names of fruit crops form a separate terminological system based on one-word fruit plant names. Names of new introduced fruit crops usually are borrowed from the language of that country where the crop has extended and whence it has spread, or on the basis of a Latin generic name. Conclusions. The registered varieties of fruit plants in Ukraine belong to 48 crops. Formation of names of fruit crops has begun in ancient times and is continuing until now. Crop names, as a rule, consist of one word; two-word names occur as an exception, if so, an adjective should be places ahead of a noun. Names of fruit crops and the name of botanic taxa are specific, belong to different terminological systems and perform different functions.

Purpose. To define morphological peculiarities of the different types of shoots of switch grass (Panicum virgatum L.) in the context of introduction in the Right-Bank Forest-Steppe and Polissia zones of Ukraine. Methods. Morpho-physiological and ontogenetic analysis, morphometric technique, statistical evaluation. Results. It was found that P. virgatum plants were producing the following types of shoots: vegetative-generative short or long rhizomatous ones (they appeared as in the underground part of a maternal shoot in «the area of shortened internodes» and in the base of its aboveground part); vegetative underground and aboveground ones (they are forming both in the axils of scales of the underground part of a maternal shoot and in the axils of cauline leaves); generative aboveground shoots (borne in the axil of cauline leaves). Morphological parameters and features of morphogenesis of reproduction, resting and secondary buds have been described. Conclusions. During flowering phase of maternal shoot, P. virgatum reproduction buds were on the organogenesis phase I–II, their bursting occured after maternal shoot has flowered. The correlation of internodes length, leave blades and sheaths has been established. The maximum values of the above parameters were observed in the middle part of the prefloral zone of the stem. In the context of introduction, panicle earing came in the third decade of July, flowering began in the first decade of August. It was revealed that complete flowers are bloomed faster than the staminate ones.

Purpose. To assess introduced samples of drought-resistant species of perennial grasses, select a promising parent material and create on its base high-yielding varie­ ies with economic characters. Methods. Field experiment, laboratory testing. Results. The results of studies on introduction and breeding were given aimed to improve drought tolerance of non-traditional perennial grasses under the conditions of the Right-Bank Forest-Steppe zone of Ukraine. Based on the selected parent material, varieties were created by the use of hybridization and ecotype breeding methods and then entered into the State Register of plant varieties suitable for dissemination in Ukraine, among them: intermediate wheatgrass (Elytrigia intermedia (Host) Nevski) – ‘Hors’, crested wheatgrass (Agropyron cristatum (L.) Gaertn.) – ‘Petrivskyi’; meadow brome (Bromus riparia Rehm.) – ‘Boian’; slender wheatgrass (Roegneria trachycaulon (Link) Nevski) – ‘Co­umb’. As compared with conventional, relatively drought-tolerant species of smooth brome (Bromopsis inermis (Leyss.) Holub) – ‘Mars’, increment of dry matter content of these species in the extreme drought conditions of 2011 was increased by 1,52–3,73 t/ha. Under more sufficient moistening conditions of 2012, slender wheatgrass ‘Columb’ was at the level of the ?heck variety in terms of this indicator. Other varieties exceeded it by 1.44–3.22 t/ha. The data was given including seed productivity and sowing quality indicators, after-ripening duration and economic fitness of seeds. Conclusions. The use of the recommended varieties of drought-resistant species of perennial grasses as part of grass mixtures will increase significantly the productivity of grasslands and pastures in the current context of climate change.

?urpose. To investigate inheritance of “plant height” trait in populations of the first (F1) and second (F2) generations of the soybean (Glucine max (L.) Merrill) and evaluate hybrid combinations in order to identify phenotypes with the highest level of heterosis for plant height. Methods. Laboratory test, mathematical and statistical analysis. Results. The inheritance of plant height in soybean was investigated. Hybrid combinations were assessed for the ability to select phenotypes with an optimal height. Different types of inheritance of this trait were found in populations of the second generation. The highest degree of heterosis for plant height was expressed by such soybean hybrids of the second generation as ‘???? ’/‘Cherniatka’ (102.6%), next were ‘? 427/‘??rado’ (36,1%) and ‘Yelena’/‘Vizhion’ (32,0%). For the above trait, the ‘Legend’ variety in combinations with such varieties as ‘Staroukrainka’, ‘Korado’ and ‘Medeia’ expressed a heterosis amounting to 28.1%, 8.3%, 6.1% accordingly. Plant height was inherited mainly in terms of negative overdominance (34.2% combinations). There were 21.1% of combinations that had a negative semidominance. Conclusions. Among large majority of crossing combinations, forms were revealed that differed greatly for the plant height. The highest variability was observed in combinations such as ‘Legenda’/‘Korado’, ‘Ustia’/‘Vizjion’, ‘?894’/‘Vizjion’, ‘Ug-30’/‘Vizjion’, ‘Legenda’/‘Annushka’, ‘?441’/‘Gentleman’, ‘?441’/‘Vizjion’, ‘Yug-30’/‘Gentleman’, ‘Angelica’/‘Annushka’ and ‘Angelica’/‘Gentleman.

Purpose. To identify and describe the basic criteria for studying and assessing decorative and economic-and-biological characters of the genus Heuchera L. varieties in the context of introduction. Methods. Introduction procedure, analytical approach, morphological analysis and biometric data evaluation. Results. Aided by the comparative morphological analysis of introduced varieties of the genus Heuchera, significant differences in their characters were revealed, including plant height, height and width of the basal rosette of leaves, generative shoot height. There was a significant variation of such parameters as the number of generative shoots within one plant, the number of flowers on a single generative shoot, flowering abundance. Based on investigation findings, 17 groups of varieties were identified for the dominant color of adaxial surface of the leaf blade and 7 groups – for inflorescence color. According to phenological observations, Heuchera varieties groups were determined and the dates of commencement and duration of plants flowering were registered in the context of introduction. The results of these investigations are the necessary basis for the study and evaluation of this culture assortment, they are important in breeding and landscaping. Conclusions. Based on the results of investigations of morphological features of introduced species of the genus Heuchera, traits and parameters for varieties grouping were defined as well as decorative and economic-and-biological traits that should be evaluated.

Purpose. To study morphoagrobiological and adaptive properties, level of yielding capacity of recently registered soft winter wheat varieties of various ecological groups under agroecological conditions of Kirovohrad variety testing station. Methods. Field study, laboratory test, analytical procedure and statistical evaluation. Results. It was established that the yield level of is a key composite indicator of genotype adaptation to agroecological growing conditions. Experimental data indicate significant deviations of yield depending on the genotype and the year of study. During three years of experiments, yield depending of the variety ranged from 4.26 to 9.71 t/ha, such varieties as ‘CN Kombin’, ‘Estivus’, ‘Tradytsiia odeska’, ‘Mudrist odeska’, ‘Lil’ and ‘Fabius’ had higher yields. In case of dry weather conditions and unfavorable agro-ecological factors, the following varieties as ‘Mudrist odeska’, ‘Veteran’, ‘Lil’, ‘Tsentylivka’, ‘Fabius’, ‘Patras’, ‘Montrei’ have demonstrated good adaptive properties. Their yield has decreased by 9,2–19,0%, while in the varieties ‘Mahistral’, ‘Poltavka’, ‘Harantiia odeska’ and ‘Pokrova’ – by 34.4, 42.4, 45.2 and 50.6% accordingly. Conclusions. Investigated soft winter wheat varieties differ in morphoagrobiological characteristics, productivity, height, maturation period, adaptability as well as economic and agronomic value. According to the complex of such indices as productivity, agronomic characters and properties as well as adaptability, in the microzone of Kirovohrad variety testing station it is advisable to grow varie­ties ‘CN Kombi’, ‘Pokrova’, ‘Mudrist odeska’, ‘Veteran’ and ‘Lil’.

Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, laboratory test, summarizing, analytical approach. Results. Potato varieties entered in the State register of plant varieties suitable to dissemination in Ukraine and new ones especially to be bred at the Institute of Potato Growing of NAAS were studied during the period of 2005–2016 for such basic economic characters as consumptive quality of tubers, content of starch, dry matters, protein, sugar, vitamins, carotenoids and mineral substances as well as aminoacids, color of flesh, suitability for industrial manufacturing of potato products and for purpose of technology. Attention was paid to the good prospects to use varie­ties with purple, blue and red potato tuber flesh with high antioxidant capacity. Potato varieties with above cha­racteristics and their complex combination were defined and described. The requirements of processing industry for potato as a raw material for manufacturing of potato food were given. Conclusions. The major criterion for consumptive qualities of a potato variety and correspondingly division for the commercial use is consumptive quality of tubers, especially content of essential nutrients and their favorable combination, improved taste and cooking quality, high antioxidant capacity, suitability for potato products manufacturing and use for purpose of technology. Potato varieties can be divided for economic purposes into edible, suitable for potato products manufacturing, technical and multipurpose ones.

Purpose. To identify the productivity of the genus Salix L. plants and study the relationship between its structural elements. Methods. Field study, laboratory analysis, analytical approach. Results. It was found that the height of a three-year plants of species ranged from 86.0 to 775.1 cm, of hybrid forms – from 197.0 to 488.0 cm. Average diameter of three-year plants varied in the range of 19.10 to 52.94 mm (species) and from 28.04 to 49.23 mm (hybrid forms). The highest stability for complex of morphological characters was observed in bog willow samples. It was determined that among species basket willow (16.94 t/ha) and white willow (21.19 t/ha) were the most productive for dry biomass yield per 1 hectare, among hybrid forms – purple willow × basket willow (23.36 t/ha) and basket willow × bog willow (18.57 t/ha). It was established that the value of the plants productivity was characterized by moderate, significant and close correlations with the average diameter of plants, length and number of shoots of the second order. Conclusions. A comprehensive assessment of productivity traits of three-year plants from willow collection was conducted, index of dry matter yield per 1 hectare was defined. The links between quantitative traits that characterize the contribution of some of them in productivity index were investigated. Method of cluster analysis was used to group samples for the similarity of complex agronomic characters. Basket willow and white willow as well as such hybrid forms as basket willow × bog willow and purple willow × basket willow were recommended to use as a source material for selection of samples with high productivity.