Objective: The present study deals with the qualitative identification of phytochemical constituents from Cucumis melo Linn fruits and seeds Method: The methanolic extract of both fruit and seed was subjected to phytochemical screening by qualitative and HPTLC methods. Outcome: The analysis revealed that the extract of Cucumis melo Linn is rich in phytochemical compounds like Alkaloids, Essential oils, Flavonoids, Phenolic compound, Steroids, Tannins and Triterpenes. The qualitative HPTLC analyses of the methanolic extract of the Cucumis melo fruit and seed extract showed the presence of Alkaloids, Essential oils, Flavonoids, Phenolic compound, Steroids, Tannins and Triterpenes .Further research is warranted for screening the biological activities of these extracts.

Plan:An analytical method for the simultaneous determination of Atorvastatin and Aspirin in capsule dosage form using 1.5M urea as hydrotropic solubilizing agent is described. Prologue: The method is simple, fast, & accurate and precludes the use of corrosive solvents and can be used for the routine analysis of commercial combinations of Atorvastatin and Aspirin. Methodology:The developed method used the simultaneous equation method (method-A)using 243 nm and 233 nm as absorbance maxima for ATR and ASP respectively and Q-absorbance ratio method (method-B), which is based on the measurement of absorptivity at iso-absorptive point 239 nm and 243 nm (absorption maximum of Atorvastatin). The calibration curves for both drugs were found to be linear in the concentration range of 10-50µg/ml. Outcome:The proposed method has been applied successfully for the simultaneous determination of Atorvastatin and Aspirin in capsule dosage form. The mean recovery of the drugs from the combination tablets was found to be 98.83 %for Atorvastatin and 97.77 % for Aspirin for method-A and 98.09 % and 98.06 % for method-B respectively. No significant interference was observed from urea and other excipients commonly used in the formulation.

Plan: we have developed new a method for quantitative estimation of Rutin hence no UV spectroscopic method is available for the quantitative analysis of rutin for bulk analysis. Preface: U.V Spectrophotometric method has been widelyemployed for determination of analyte in a mixture. Our aim is to developspectroscopic method for estimation of the Rutin m in ternary mixture by usingU.V spectrophotometry. Methodology: The method was validated as per ICH guidelines. The recovery studies confirmed the accuracy and precision of the method. Outcome: It wassuccessfully applied for the analysis of the drug in bulk and could beeffectively used for the routine analysis.

Plan: Dexchlorpheniramine maleate is a Chlorpheniramine dextrorotatory isomer, which is twice active than Chlorpheniramine. It’s widely used in the coughs, cold and allergic conditions including angioedema, urticaria, conjunctivitis, rhinitis, and pruritic skin disorders. The main objective of the present research work deals with the development of simple, rapid and sensitive RP- UFLC analytical method for estimation of Dexchlorpheniramine maleate in bulk and finished formulation. Methodology: simple, rapid and sensitive RP- UFLC analytical method was developed. Optimized chromatographic separation condition was done by using an isocratic mode with the mobile phase of 25 mM of potassium dihydrogen ortho phosphate (pH 4) and acetonitrile at the ratio of 80:20v/v with a flow rate of 1ml/min. Stationary phase used was C18 column (250 x 4.6 mm). Dexchlorpheniramine maleate detection was carried at 260 nm. The developed method was evaluated for the validation parameters as per the ICH guidelines. Outcome: The developed optimized chromatographic condition was achieved and results showed good peak resolution. The developed method for the Dexchlorpheniramine maleate can be used for the qualitative and quantitative estimation of finished and bulk formulations.

Plan: Several authors have reported the presence of alarmingly high levels of heavy metals like lead, arsenic, cadmium and mercury in ayurvedic medicines. Ayurveda employs several classes of medicines, among which gulika (tablets) are an important one. However, it is not known whether traditional ayurvedic pills are free from heavy metals. The present study was therefore, undertaken to study the heavy metal content of some representative ayurvedic tablets manufactured using herbs. Methodology: Samples of ayurvedic tablets were obtained from the market and the content of lead, arsenic, cadmium and mercury was determined using ICP-MS. Outcome: Among the 16 Ayurvedic tablets analyzed, 4 failed for lead, 2 for arsenic and 6 for mercury. All the tested samples had cadmium below the limit set by Government of India. A review of the available reports and the present study support the contention that tablets are the worst affected ayurvedic dosage form. Urgent measures are to be taken to control this problem.

Ayurveda dates backat least 2,000 years in its codified form. However, it has roots that are muchdeeper. The present day Caraka Samhita,Susruta Samhita and A??a?gah?daya refer to ancient and long-losttreatises like Nimi Tantram and Kharanadi. Nevertheless, in the absenceof authentic records it is difficult to prove the antiquity of Ayurveda. In thelate twentieth and early twenty-first centuries, Ayurveda is traversing wellbeyond the boundaries of its homeland. It is fast becoming a transnational andmulticultural phenomenon. It is the fastest growing alternative medical systemin German - speaking Austria, Germany and Switzerland. The theory andpractice of Ayurveda revolve around the trido?a doctrine,which states that all biological activities in the human body are controlled bythree factors- vata, pitta and kapha, collectively called trido?a. Dietaryand behavioural indiscretions and seasonal climatic factors causedestabilization of the trido?a,which slowly progresses through four stages (cayam, prakopam,prasaram, sthanasamsrayam). This progression results in well-defined diseaseentities (vyakti) like jvaram, atisaram, graha?i, pa??u, arsas etc. However, if left untreated, the disease progresses tothe sixth and last stage (bhedam) at which it may or may not becurable. Ayurveda calls upon individuals to consume wholesome food and adoptdaily and seasonal regimen, so that the trido?aremain in steady state. Many diseases are said to appear on account of the evilactions committed in present and previous lives. Therefore, it is essential toindulge in ethical activities and to refrain from committing unethical acts, ifone wants to lead a disease-free life.........