Abstract :Periodontal tissues are predominantly formed by ecto-mesenchymal cells derived from the neural crest during embryonic development. Neural Crest Cells (NCCs), a transient multipotent stem cell population that plays crucial roles in the tissue development, have been regarded as highly promising candidates for the periodontal tissue regeneration. Our previous study demonstrated the establishment of a multipotent clonal Periodontal Ligament (PDL) cell line termed cell line 1-17 that showed NCCs phenotypes. In addition, our studies also reported the generation of Neural Crest-Like Cells (NCLCs) from human PDL-derived Induced Pluripotent Stem Cells (iPSCs). This article discusses the application of cell line 1-17 and human PDL iPSC-derived NCLCs for the study of clinical periodontal therapy.