VPH infection and its clinical correlation in indigenous population of the high andean region of Ecuador | Author : Yenddy Carrero, Elizabeth Proaño-Pérez, Fernanda Vasquez, Alex Armijos, Carmen Viteri-Rpbayo, Alicia Zabala-Calahorrano | Abstract | Full Text | Abstract :Cervical-uterine cancer (CaCu) is a public health problem. Epidemiological studies have identified a close relationship between CaCu and human papillomavirus (HPV) infection. The purpose of this study was to perform the molecular detection of HPV, identification of mixed infections and the screening of CaCu through conventional cytology in an indigenous population of the high Andean highlands of Ecuador in order to correlate the data obtained with the presence of lesions. Methodology: A cross-sectional study was carried out on 189 women between the ages of 21 and 65; who belonged to the community of Pilahuin in the province of Tungurahua, who assisted to gynecological check-ups. Data were analyzed with the EPI-INFO and SPSS statistical programs. Results: the average age of the participants was 35.2 years, most of the participants identified themselves as indigenous, in a stable relationship, multiparous, with a medium to low level of education and no paid work. The participants showed a favorable attitude towards the cervico-uterine cytology in 100% of the cases without showing a relation with age, level of education or civil status. HPV infection was positive in 10.5% of the participants, 70% of the patients had severe inflammation with a positive association with mixed bacterial infection |
| Distribution of species and antimicrobial susceptibility in Enterococcus strains of clinical origin | Author : Maribel Josefina Castellano-González, Armindo José Perozo-Mena, Kimberly Gutierrez, Jessica Jimenez, María Urdaneta | Abstract | Full Text | Abstract :In order to establish the frequency of isolation of the different species of enterococci, their distribution according to the type of sample and patient care service and determine the antimicrobial resistance, 1,624 strains obtained from bacteriological cultures of patients attended in the Bacteriological Reference Center at the Autonomous Service University Hospital of Maracaibo, during the period January 2010 - December 2015, were analyzed. The most frequent species were E. faecalis (52.46%) and E. faecium (41.38%). The greatest number of strains was obtained from skin and soft tissues samples (54.92%), urine (23.15%) and blood (17.27%). Services with increased frequency of isolation were: hospitalization of adults (47.71%) and pediatric hospitalization (16.38%). It did not find statistically significant association between the specie of enterococci and sample type, or patient care service (p > 0.05). It was detected more resistance in E. faecium than in E. faecalis. The enterococci are acquiring ever greater antimicrobial resistance, and therefore, it is necessary to maintain permanent vigilance over them, perform their proper identification and timely detect resistance, in order to apply preventive measures before these microorganisms cause a greater intrahospital impact |
| Microbiological quality of raw salads that are sold at roadside food stalls quick Maracaibo-Venezuela | Author : Albanys Delgado Gómez, Lisette Sandrea Toledo, Giovanna Bonfini Quintero, Yadira Higuera Donado, Yeiny Avila Roo, Kutchynskaya Valero Leal | Abstract | Full Text | Abstract :Raw salads play an important role in foodborne, especially during processing is not necessary meet to prevent its contamination hygienic conditions. The objective is to determine the microbiological quality of raw salads that are sold in fast food stalls Maracaibo, Venezuela. 15 establishments were studied by double sampling interval of one month. For microbiological study, samples were prepared according to the Venezuelan Industrial Standards Commission (COVENIN No. 1126-1189). Homogenate, counting mesophilic aerobics, Salmonella, Total and Fecal Coliforms and Escherichia coli by the method most probable number and rehydratable plate with dry films was determined. Regarding mesophilic aerobics, of the 30 samples, 2 (6.67%) showed counts of 225,000 CFU/g; and 28 (93, 33%) had countless counts. For Total Coliforms, 28 (93.3%) showed counts ranging from 350 to 730 x107 x 107 and 2 (6.6%) the average count was 197 CFU/g. Escherichia coli in 93.3% (28) of the samples was observed high counts, and in 2 (6.6%) counting was less than 1 CFU/g. Salmonella was detected in 4 (13.3%) samples. The salads studied are unfit for human consumption, not to meet the minimum safety requirements, according to established regulations |
| Knowledge about healthcare associated infections in postgraduate residents from a venezuelan university | Author : Armando Guevara, Abraham Figuera | Abstract | Full Text | Abstract :A descriptive, cross-sectional study was carried out with field design to evaluate the knowledge about the healthcare associated infections (HCAI) and its prevention in postgraduate residents of the School of Health Sciences "Dr. Francisco Battistini Casalta” of the Universidad de Oriente, Venezuela. The sample consisted of 51 residents’ physicians from different postgraduate programs. A survey was applied to investigate knowledge on three specific areas: generalities about HCAI, standard precautions and hand hygiene. They were considered to have adequate knowledge if they obtained an average score of 17.5 points or more. 58.8% of the respondents were female with an average age of 31 years (SD ±4.32, Range: 25-47). The average score in the survey was 17.9 points (SD ±1.87, Range: 12-22). The 56.87% approved the survey. When evaluating the knowledge about the studied areas, it was found that the majority have basic concepts about standard precautions (86.27%) but not about generalities of IAAS (37.25%) or hand hygiene (19.6%). In conclusion, the postgraduate residents have adequate knowledge about the HCAI and its prevention but present deficiencies in specific aspects |
| Cytological findings and risk factors associated to cervical lesions in indigenous women belonging to three etnias of Zulia State | Author : Yenddy Carrero, Angela Bracho, María Arguello, C Silva, MJ González, D Marín, R Atencio | Abstract | Full Text | Abstract :The cervical cancer represents a problem of public health and is considered the second reason of death by cancer in women. There have been described factors associated, related to the conduct, habits and infectious agents, such as Human Immunodeficiency Virus, herpes virus, Chlamydia trachomatis and Human Papilloma Virus (HPV). In order to establish risk factors and detect preinvasive cervical lesions, 101 samples of women from three indigenous ethnic groups of Zulia State were selected; to which a clinical-epidemiological survey was carried out to study the predisposing factors, in addition to the cytological study. Of the total of patients studied 33.66% were negative to the cytological study, while 66.34% had some type of alteration, observing that the Yukpa the most affected, 21 (20.79%) presented Cervical intraepithelial neoplasia and related infectious agents were found in 56.51% of the women. Coelocytic cells suggestive of HPV were found in 26.21% of all patients. When we analyzed the risk factors, we found that, the onset of sexual intercourse before age 15 as well as alcohol consumption were the most relevant. According to the findings, it is important the timely diagnosis in these communities, which are disassociated, and without access to health centers, whether due to living conditions or cultural implications |
| Infección urinaria en pacientes con diabetes mellitus tipo 2: frecuencia, etiología, susceptibilidad antimicrobiana y factores de riesgo | Author : Elsa Lucas-Parrales, Cristobal Franco, Maribel Castellano-González | Abstract | Full Text | Abstract :Las infecciones urinarias son comunes en pacientes diabéticos. El objetivo de esta investigación fue: determinar la frecuencia, etiología, susceptibilidad antimicrobiana y factores de riesgo asociados a infección urinaria en pacientes con diabetes tipo 2. Se estudiaron 108 pacientes ambulatorios, con diagnóstico presuntivo de infección de vías urinarias, durante el periodo mayo 2016 - mayo 2017, en la ciudad de Jipijapa, Ecuador. Para el urocultivo se utilizó la técnica del asa calibrada. La susceptibilidad a los antibióticos se determinó mediante el método de Kirby & Bauer. Las variables cualitativas se compararon con ji cuadrado o la prueba exacta de Fisher. Se calculó el Odd radio (OR) y sus intervalos de confianza (IC) del 95%. Valores de p = 0,05 fueron considerados significativos. La frecuencia de infección urinaria fue de 73,15%. El microorganismo más frecuentemente aislado fue Escherichia coli (78,48%). Los mayores porcentajes de resistencia se observaron para amoxicilina (78,87%) y cefalexina (71,83%). Los malos hábitos de higiene, la presencia de cálculos renales y una vida sexual activa resultaron factores de riesgo para las infecciones urinarias. La nitrofurantoina, fosfomicina, fluoroquinolonas y algunos betalactámicos, todavía representan una alternativa de utilidad en la quimioterapia de las infecciones urinarias no complicadas en pacientes diabéticos |
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