Abstract :Abstract
Simple and efficient syntheses of NAD+, an important biological cofactor, and its precursors have been limited to amido versions. Here we report the novel synthesis of two previously overlooked NAD+ precursors, NaMN and NaAD, using a common intermediate, ethyl nicotinate riboside (O-ethyl-NaR). In addition, these synthetic metabolites were shown to be substrates for NAD+ biosynthetic enzymes and were sufficient to increase cellular NAD+ levels.