Abstract :Abstract
Phospholipids are important class of complex molecules that provide structural and functional roles
in the biological systems. Phospholipids anomalies and disorders are generalized membrane defects
and so can be detected in a variety of tissues, including body fluids and blood elements. Highperformance
liquid chromatographic method for the analysis of major phospholipids is reported.
Isocratic separation of phospholipids was achieved on silica gel column using elution solvent
mixture of acetonitrile-methanol-phosphoric acid (100:10:1.8. v/v) within 9.5 min. Detection
of phospholipids was attained by UV spectrometer at 203 nm with detection limit ~5 ng. The
present method of HPLC hyphenated-UV system offers advantages of high speed and simplicity
for the separation and detection of a variety of phospholipids including phosphatidylserine,
lysophosphatidylserine, phosphatidylcholine , lysophosphatidylcholine, phosphatidylethanolamine,
lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidic acid and
sphingomyelin in routine clinical studies pertaining to membrane/ cell disorders in health, toxicity,
diseases, and biological systems, and in basic research, as well.