Abstract The purpose of this study was to assess the safety use of Murraya koenigii in terms of repeated dose toxicity study in male and female rats. Three doses 300, 500 and 900 mg/kg of ethanolic extract of Murraya koenigii leaves were administered to groups of 10 animals (five male and five female) daily for 28 days respectively. Animals receiving the vehicle (0.5% CMC) served as a control. The biochemical parameters like RBC, WBC, glucose, hemoglobin, cholesterol, creatinine, bilurubin, SGPT and SGOT were analyzed in all groups of animals respectively. While in physiological parameters like daily food consumption, weekly body weight, visual, auditory and organ weights were analyzed respectively. At the end of study all the animals were sacrificed and tested for histopathology for any structural damage to major organs. Result of study showed that none of the animals from any dose levels showed test material related changes in RBC, WBC, creatinine, bilurubin, SGPT and SGOT parameter respectively. Similar results showed for some physiological parameter like daily food consumption, visual and auditory activity. There were no other changes except increased in hemoglobin, drastic and sudden weight loss, decrease in cholesterol and glucose of these animals. Animals from 500 mg/kg and 900 mg/kg showed loss of subcutaneous fat during last two week of treatment. At the same dose level there decreases in total cholesterol and glucose level. While the significant effect was observed with at dose of 900 mg/kg. The Histopathological study showed mild congestion in all treatments groups while haemorrhage and lymphocyte infiltrate observed at 900 mg/kg groups of male and female rats. From results it conclude that administration of ethanolic extract Murraya koenigii leaves for 28 days increases hemoglobin level, decreases the body weight, subcutaneous fat and blood glucose level, as observed in medium and high dose group. Murraya koenigii do not show any structural damage to major organs except lymphocyte infiltration and haemorrage.