Amino acids, soluble sugars and peroxidase activity in anonaceas cutting collected in two years | Author : Cristiano Pereira da Silva, Elizabeth Orika Ono, João Domingos Rodrigues, Luiz de Souza Corrêa, Aparecida Conceição Boliani | Abstract | Full Text | Abstract :In order to guarantee better results in the rooting of cuttings of some fruit species, it is necessary to determine the best time for the removal of the branches and making the cuttings that will be used as vegetative propagules in the method of propagation by cuttings. Apical and basal pinheira (Annona squamosa L.), gravioleira (Annona muricata L.) and atemoeira (Annona cherimola L. x Annona squamosa L.) cuttings were collected at two seasons of the year, containing branch mix, making the samples for analysis. These samples were ground and processed in the laboratory to perform the biochemical analyzes, in order to verify the total amino acid concentrations, sugars and enzymatic activity of the peroxidase. These cofactor substances aid in the best rooting of cuttings. Among the results the best season for the removal of the branches for propagation by cutting is in the summer period, both for the apical and basal cuttings of the species of anonas analyzed. |
| Prevalence of intestinal parasites in domestic and wild birds of the south of Brazil | Author : Sandra Márcia Tietz Marques, Luiza de Campos Menetrier, Ana Carolina Contri Natal, Laura Souza Fernandes, Jacqueline Meyer, Marcelo Meller Alievi | Abstract | Full Text | Abstract :The objective was to monitor and know the parasite diversity of domestic and wild birds through coprological exams. Fecal samples were obtained from the Center for Rehabilitation and Conservation of Wild Animals (Preservas) of the Faculty of Veterinary Medicine of the Federal University of Rio Grande do Sul, Brazil. The collections were executed for three years (2015 to 2017), composed of faeces of 203 birds distributed in 21 Orders. The faeces were processed by the methods of Willis-Mollay and Lutz. The total prevalence was 38.8% (80/206) with 45.8% (38/80), 40% (26/80) and 27.6% (16/80), respectively, in 2015, 2016 and 2017. The fecal collection routine is relevant for the identification on the parasitic fauna of wild birds. The inclusion of parasite treatment assists in the rapid recovery of birds when there are competing pathologies. |
| Liquid-liquid extraction by low-temperature partition and high-performance liquid chromatography for determination of flunixin and phenylbutazone in horse hair | Author : Fernando Luciano Alves de Souza, Andréa Alice da Fonseca Oliveira, Ana Paula Silveira Paim | Abstract | Full Text | Abstract :This work proposes the determination of flunixin and phenylbutazone in horse hair using liquid-liquid extraction by lowtemperature partition (LLE-LTP) and HPLC-UV detection. The method efficiently extracted the analytes and purified the
extract in a single step. The method has never been applied for horse hair and showed to be easy to perform with satisfactory
results. Limits of detection and quantification were 0.012; 0.024 ng mg-1 for flunixin and 0.036; 0.073 ng mg-1
for phenylbutazone, respectively. Nine out of 14 samples analyzed showed positive results for at least one analyte. The average recoveries for flunixin and phenylbutazone were above 97.0 and 92.0 %, respectively. |
| First report of Meloidogyne javanica on potato (Solanum tuberosum L.) in the southern region of the state of Goiás | Author : José Feliciano Bernardes Neto, João Pedro Elias Gondim, Nadson de Carvalho Pontes, Rodrigo Vieira da Silva | Abstract | Full Text | Abstract :Potato tubers (Solanum tuberosum L.) exhibiting symptoms of nematode attack were located in a planted area in the
municipality of Morrinhos-GO. Thus, in order to clarify the etiology of the disease, some plants were collected. The roots
and tubers were analyzed, which presented typical galls, with the appearance of superficial "popcorn" in the tubers and,
when dissecting the galls, white-milky females were observed, typical symptoms and signs of root-knot nematodes, genus
Meloidogyne. Part of the roots was used to extract eggs for multiplication in greenhouse tomatoes. Female perineal pattern
and isoenzyme (esterase) electrophoretic pattern analysis confirmed the species Meloidogyne javanica. This is the first
report of M. javanica infecting the potato crop in the southern region of the state of Goiás, serving as an alert to potato and
vegetable farmers in general, in order to prevent the spread of the nematode in other areas of planting. |
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