Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution | Author : Melly Agustin | Abstract | Full Text | Abstract :The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Hellys fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution. |
| Incompatible Results on Matched Cross Test Examination | Author : Nur Fajrin Aljannah, Francisca Romana Sri Supadmi | Abstract | Full Text | Abstract :Compatibility testing before blood transfusion is very crucial. This procedure to prevent transfusion reactions due to blood group incompatibility or irregular antibodies. Cross-matching to ensure that the blood safe for the recipient. There are two results in the cross-matching, namely compatible and incompatible. This study aims to determine the inappropriate results of cross-matching at Blood Transfusion Service of the Indonesian Red Cross, Kulon Progo Regency. This study used a quantitative descriptive research design with a retrospective approach. The samples used in this study were 78 incompatible samples in Blood Transfusion Service of the Indonesian Red Cross, Kulon Progo Regency. The sampling technique used was the total population. Methods of data analysis using descriptive analysis. The results show incompatibilities of most compatible cross tests found in minor and auto control (96.1%) with male gender frequency (35.9%) and women (64.1%). The most blood type that has incompatibilities is blood type O (43.3%). The most Diagnosis is anemia (76.9%), and the most components are PRC (88.4%). The most incompatible types are minor and auto control and the most commonly encountered in women, O blood type, anemia, and components of PRC.
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| Wound Closure Ratio in Streptozotocin-Induced Diabetic Mice Treated by Passive and Interactive Dressing (Pilot Study) | Author : Eko Naning Sofyanita, Arya Iswara | Abstract | Full Text | Abstract :Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol. |
| Hemoglobin Levels in Blood Samples for Public Fuel Filling Station Officers | Author : Rosalinda Zakhwa | Abstract | Full Text | Abstract :Hemoglobin is a part of erythrocytes that serves to transport oxygen and carbon dioxide in tissues. Hemoglobin levels in the blood may decrease one of which is influenced by exposure to lead from the environment. Places that are at high risk for exposure to air lead one of them is at gas stations (Public Refueling Stations). The purpose of this study is to determine hemoglobin levels in kedungrandu gas station officers, Kec. Patikraja, Banyumas. This type of research is a non-experimental observational study. The method of measuring hemoglobin levels using strip test in the form of POCT (Point Care of Testing) tool. This method uses capillary blood samples from 20 respondents with criteria of male sex and aged 21-60 years. The result of this study was as many as 6 people (30%) of the total respondents had low hemoglobin levels, while 14 people (70%) have normal hemoglobin levels. The average hemoglobin level in the blood sample of kedungrandu gas station officers, Kec. Patikraja, Banyumas is 14.41 mg/dl or normal. |
| Uric Acid Levels in Type 2 Diabetes Mellitus Patients | Author : Linda Ramadhanti, Devi Etivia Purlinda | Abstract | Full Text | Abstract :Patients with type 2 diabetes mellitus are about 90% of all cases of DM in the world. Type 2 diabetes is caused by the bodys inability to respond properly to insulin or is called insulin resistance. Insulin resistance causes hyperglycemia and hyperinsulinemia which results in decreased function of uric acid excretion in the kidney tubules, and causes an increase in uric acid in the blood or hyperuricemia. This type of research is descriptive, with a cross sectional approach in May for 5 days from May 17 to May 21 2019. The research sample is type 2 DM outpatients, at Dr. Hospital. Adhyatma MPH consisted of 24 people who were taken based on total sampling who agreed to the informed consent, were over 20 years old, had no history of gout, and fasted for 10-12 hours. The purpose of this study was to determine the uric acid levels of type 2 DM outpatients based on gender, age, and blood pressure at Dr. Hospital. Adhyatma MPH. The results showed that normal uric acid levels in type 2 DM outpatients were 14 people (58.3%) and hyperuricemia 10 people (41.6%). 7 patients (29%) each had normal urate levels. The highest normal uric acid levels were in patients aged over 55 years as many as 9 people (37.5%) and 10 people with hypertension (41.7%). The most hyperuricemia occurred in 6 female patients (25%), 6 patients over 55 years old (25%) and 6 patients with hypertension (25%). |
| Levels of Cadmium (Cd) in Urine in Passive Smokers | Author : Nurhayana, Anung Sugihantono | Abstract | Full Text | Abstract :Smoking is a familiar activity in daily life. The existence of filters on cigarettes, causes the danger of cigarette smoke to be felt more by passive smokers. One of the content of cigarette smoke is cadmium (Cd) which is one of the environmental pollutants that are harmful and accumulate in the tissues of living things. The purpose of this study is to measure and describe the levels of Cadmium (Cd) in the urine of passive smokers in RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen District, Semarang Regency. Using observational (non-experimental) research design with descriptive research criteria. The number of samples of 12 urine from the community of RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen Subdistrict, Semarang Regency. The results of the examination of Cadmium Levels (Cd) on the 13 samples of passive smoker urine in gandekan RT 01 RW 06 Harjosari Subdistrict, Bawen Subdistrict, Semarang District received the highest level of 0.074 mg/L and the lowest result -0.005 mg/L. Differences in cadmium levels in the passive smoker urine were caused by several factors such as age, environment, health status, occupation, and consumption of antioxidants and milk. Cadmium (Cd) levels in the urine of all passive smokers were in the normal category (<2mg/L).
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| Uric Acid Levels in Menopausal Women | Author : Safira Zahro, Umi Rosidah | Abstract | Full Text | Abstract :Based on data from Riskesdas 2013, in line with increasing age the prevalence of joint disease has increased. The trigger is abnormal metabolic processes in the body and 10% of cases occur in postmenopausal women because the production of the hormone estrogen is disrupted. If treatment is not carried out, it will result in chronic gouty arthritis, resulting in paralysis. The purpose of this study was to determine the description of uric acid levels in postmenopausal women in Berahan Kulon Village, Wedung District, Demak Regency. The research method used is an observational study with a cross sectional approach. Of the 20 research subjects, 1 research subject had low uric acid levels, 9 research subjects had normal uric acid levels, and 10 research subjects had high uric acid levels. High uric acid levels were dominated by the age group 51-55 years, menopause duration 6-10 years and > 10 years, no history of heredity, and consumption of foods high in uric acid 3-5 times/week and > 5 times/week. The conclusion of this study is that 5% of postmenopausal women have low uric acid levels, 45% have normal uric acid levels, and 50% have high uric acid levels. |
| Inter Trimester Hemoglobin Levels in Pregnant Women | Author : Bella Andira Safitri | Abstract | Full Text | Abstract :Hemoglobin functions to transport all oxygen from the lungs to the tissue cells. Hemoglobin contains iron and is still present in red blood cells. In pregnant women hemoglobin levels are often abnormal. According to PMK RI No. 97 of 2014 concerning health services, it stipulates that pregnant women are checked for hemoglobin at least once in the first trimester and once in the third trimester. This is done to determine the condition of pregnant women during pregnancy. Low hemoglobin levels cause anemia. Anemia in pregnant women can affect the process of fetal growth and development in the womb. The purpose of this study was to determine the hemoglobin level between trimesters in pregnant women at the Jatilawang Health Center, Banyumas Regency in 2020. The research criteria was a descriptive study using a retrospective time series design using secondary data. The results of inter-trimester hemoglobin levels in pregnant women at the Jatilawang Health Center, Banyumas Regency in 2020 are in the first trimester 40% normal hemoglobin levels and 60% abnormal hemoglobin levels, in the second trimester hemoglobin levels are 72% normal and 28% hemoglobin levels are not normal, in the second trimester the hemoglobin levels are normal and 28% are abnormal. III 96% normal hemoglobin level and 4% abnormal hemoglobin level. Inter-trimester hemoglobin levels in pregnant women at the Jatilawang Health Center, Banyumas Regency in 2020 tend to be higher based on trimesters I, II and III. |
| Hematocrit Value in Diarrhea Patients | Author : Aulia Prameswari Hayuning Putri | Abstract | Full Text | Abstract :Diarrhea is the passage of abnormal or watery stools that are more frequent than usual, and can be caused by infection or non-infection. Complications can occur including dehydration in patients with acute diarrhea. In cases of severe dehydration, hematological tests in diarrheal patients may be elevated, such as hematocrit and hemoglobin values. The purpose of this study was to determine the hematocrit value in inpatient diarrhea patients at the Tonjong Public Health Center, Brebes Regency. This type of research is a descriptive study using secondary data from medical record data as many as 30 respondents at the Tonjong Public Health Center, Brebes Regency. The results showed that there were 3 men who had decreased hematocrit levels and 8 people who had normal hematocrit levels. Whereas. on. In women, the hematocrit level decreased as much as. 17 people and 2 people who have normal hematocrit levels. Based on the age category, the most are the late elderly as many as 8 people and the final adults as much as 2 people. Meanwhile, based on the status of dehydration, all diarrhea patients who came for treatment at the Tonjong Health Center, Brebes Regency, 100% did not experience diarrhea with dehydration. The hematocrit levels in diarrhea patients at the Tonjong Public Health Center, Brebes Regency were 30 samples, on average they had low hematocrit levels. |
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